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What (who) is 4Pi STED microscopy - definition
4Pi STED microscopy
The 4Pi-STED-microscope is the result of combining the two unrelated concepts of Stimulated emission depletion (STED) microscopy and 4Pi-microscopy. Here, a fluorescent sample is placed in the common focus of two opposing lenses, but excitation and detection are performed through a single lens (4Pi mode A).
4Pi microscope
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A 4Pi microscope is a laser scanning fluorescence microscope with an improved axial resolution. With it the typical range of the axial resolution of 500–700 nm can be improved to 100–150 nm, which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy.
